By Larry L Hench
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Extra info for Biomaterials, Artificial Organs and Tissue Engineering (Woodhead Publishing in Materials)
Submicron biodegradable polymer membrane hemoglobin nanocapsules as potential blood substitutes: a preliminary report. Artificial Cells Blood Substitutes and Immobilization Biotechnology 1994; 22:889±894. Soonshiong P, Heintz RE, Merideth N, Yao QX, Yao ZW, Zheng TL et al. Insulin independence in a Type-1 diabetic patient after encapsulated islet transplantation. Lancet 1994; 343(8903):950±951. Aebischer P, Schluep M, Deglon N, Joseph JM, Hirt L, Heyd B et al. Intrathecal delivery of CNTF using encapsulated genetically modified xenogeneic cells in amyotrophic lateral sclerosis patients.
81. Tagalakis AD. Apolipoprotein E delivery by peritoneal implantation of encapsulated recombinant cells improves the hyperlipidaemic profile in apoEdeficient mice. Biochimica et Biophysica Acta ± Molecular and Cell Biology of Lipids 2005; 1686(3):190±199. 82. AlHendy A, Hortelano G, Tannenbaum GS, Chang PL. Correction of the growth defect in dwarf mice with nonautologous microencapsulated myoblasts ± an alternate approach to somatic gene-therapy. Human Gene Therapy 1995; 6(2):165± 175. 83. Ross CJD, Bastedo L, Maier SA, Sands MS, Chang PL.
Mechanical sections of embedded particles allow inspection of the particle wall structure, but it is not possible to visualize the encapsulated phase which is often lost during the microscopy sample pre-treatment. Although the membrane thickness and coating density substantially affect the properties and performance of the microcapsules, these parameters are difficult to quantify. 175 and Ross and Chang176 found that APA microcapsules had a wall thickness of 11±13 m, whereas others reported a membrane thickness of 40±120 m177±179 using prior labeling methods.
Biomaterials, Artificial Organs and Tissue Engineering (Woodhead Publishing in Materials) by Larry L Hench