Download PDF by Minoru Ueda (Ed.): Applied Tissue Engineering

By Minoru Ueda (Ed.)

ISBN-10: 9533076895

ISBN-13: 9789533076898

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Although the cryopreserved cell sheets could not maintain their original layered structure after thawing, viable cell sheets could be regenerated. The cell survival rates obtained after freezing storage were reasonable; however, the cryopreserved sheets could not maintain their original layered structure. More works needs to be done to better preserve the corneal epithelial cell sheets. References 1. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.

Allogenic or xenogenic materials can eliminate this concern but not the risk of disease transmission. As another solution, the use of injectable tissue-engineered bone in bone augmentation procedures as a replacement for autologous bone grafts, offers predictable results with minimal donor-site morbidity [23, 65]. We considered that tissue-engineered bone is beneficial material for alveolar cleft osteoplasty, and applied this treatment. Surgical techniques Following a 3-cm-long mucosal incision at the level of the labiogingival junction, dissections were made in the ingrown scar tissue to reach the bony surface of the cleft walls.

Protocol of autologous fibroblast injections for soft-tissue augmentation. (a) Oral mucosa. (b) Autologous serum. (c) Cultured fibroblast with autologous serum. (d) Cell transplantation (From Ebisawa et al. 2008. Reprinted with permission). Chapter 1: Skin 13 A B Fig. 6. A: Before treatment. B: Three years after treatment (From Ebisawa et al. 2008. Reprinted with permission). References 1. De Luca M, Albanese E, Bondanza S. Multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium, fresh or preserved in frozen state.

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Applied Tissue Engineering by Minoru Ueda (Ed.)

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